D-(+)-Biotin (Vitamin B7)
Vitamins
Nutritional Enhancers
58-85-5
C₁₀H₁₆N₂O₃S
$99.95 ~ $149.93
Food
Free sample from 100g(NF)
One unit of:25kg/carton
One unit of:25kg/carton
25kg/carton
Product Info
What is D-(+)-Biotin (Vitamin B7)?
D-(+)-Biotin, also known as Vitamin B7, is an essential water-soluble B vitamin required for key metabolic functions, primarily used as a dietary supplement ingredient and a fortifying nutrient in foods and beverages.
How is D-(+)-Biotin (Vitamin B7) made?
| Step No. | Production Stage | Key Action | Control Point & Note |
|---|---|---|---|
| 1 | Raw Material Inspection | Verify the identity, purity, and chiral integrity of key starting materials (e.g., L-Cysteine, Fumaric Acid derivatives, and specific reagents). | Control Point: Purity analysis via HPLC, spectral analysis (FTIR), and optical rotation measurement. Note: The chiral purity of the starting material is the absolute foundation for synthesizing the correct D-(+)-isomer. |
| 2 | Thiolactone Intermediate Synthesis | Synthesize a key chiral intermediate, typically a bicyclic thiolactone, which forms the first ring of the biotin structure through a multi-step reaction sequence. | Control Point: Strict control of reaction temperature, time, and reagent addition sequence. Note: This stage establishes the critical stereocenters that will define the final molecule's three-dimensional shape. |
| 3 | Imidazolidone Ring Formation | Construct the second (imidazolidone) ring onto the thiolactone intermediate, often using reagents like phosgene or its equivalents and ammonia sources. | Control Point: Monitoring pH, temperature, and reaction completion using in-process controls (IPC) like TLC or HPLC. Note: This completes the core bicyclic ureido structure characteristic of biotin. |
| 4 | Side Chain Attachment | Introduce the five-carbon valeric acid side chain to the bicyclic core, commonly via a Wittig or similar carbon-carbon bond-forming reaction. | Control Point: Maintaining anhydrous (water-free) conditions and precise temperature control during the reaction. Note: The correct attachment and length of this carboxylic acid side chain is essential for the vitamin's biological function. |
| 5 | Stereoselective Reduction | Reduce the double bond(s) in the intermediate molecule using catalytic hydrogenation to finalize the stereochemistry. | Control Point: Catalyst selection (e.g., Palladium on Carbon), hydrogen pressure, and reaction temperature. Note: This step is highly stereospecific, ensuring the required cis-ring fusion of the two rings is achieved, which is vital for the correct isomer. |
| 6 | Purification & Crystallization | Isolate the crude D-(+)-Biotin from the final reaction mixture and purify it through repeated crystallization from a suitable solvent system. | Control Point: Solvent purity, cooling profile, agitation speed, and filtration efficiency. Note: This is crucial for removing isomers, unreacted materials, and achieving the required high purity (>99%) for pharmaceutical or food-grade applications. |
| 7 | Drying, Milling & Packaging | Dry the purified biotin crystals under vacuum, mill to a specified particle size, and package into sealed, protective containers. | Control Point: Final moisture content (<1.0%), particle size distribution (PSD), and absence of foreign matter. Note: The product is packaged in light-resistant, airtight containers to ensure stability and prevent degradation. |
Technical Specifications
| CAS Number | 58-85-5 |
| Chemical Formula | C₁₀H₁₆N₂O₃S |
| Solubility | 22 mg/100 mL water at 25 °C; 80 mg/100 mL alcohol at 25 °C; soluble in 0.1 M NaOH |
| Storage Conditions | store in cool, dry, <25 °C, protect from light and moisture |
| Shelf Life | 24 Months |
Applications & Usage
Common Applications:
dietary supplement
food/feed fortification
cosmetic ingredient
enzyme cofactor
cell culture media additive
Mechanism of action:
| Parameter | D-(+)-Biotin (Vitamin B7) |
|---|---|
| Functional Category | Nutrient; Coenzyme; Fortifying Agent |
| Key Ingredients | D-(+)-Biotin (C10H16N2O3S) |
| Mechanism of Action | Acts as a covalently bound coenzyme for carboxylase enzymes (e.g., acetyl-CoA carboxylase, pyruvate carboxylase). It functions as a mobile carboxyl group carrier, facilitating the transfer of CO2 in essential metabolic pathways, including fatty acid synthesis, gluconeogenesis, and amino acid catabolism. |
| Application Effect in Product | Nutritional fortification of foods, beverages, and dietary supplements. Supports label claims related to energy metabolism, macronutrient metabolism, and the maintenance of healthy skin, hair, and nervous system function. Prevents biotin deficiency. |
Comparison:
| Product Name | Category/Type | Key Features | Strengths (vs peers) | Weaknesses (vs peers) | Best Use Cases | Why Choose |
|---|---|---|---|---|---|---|
| D-(+)-Biotin (Vitamin B7) | Water-Soluble B-Vitamin / Coenzyme | Biologically active isomer of biotin; essential for carboxylase enzymes; supports metabolism of fats, carbohydrates, and proteins; aids in maintaining healthy hair, skin, and nails. | The only one of the eight stereoisomers of biotin that is biologically active and usable by the human body. It is the pure, standard form used in nearly all supplements and clinical studies. | Water-soluble, so the body does not store it, requiring regular intake. Very high doses can interfere with certain laboratory diagnostic tests, particularly immunoassays (e.g., thyroid function tests). | Addressing diagnosed biotin deficiency; supporting keratin infrastructure for stronger hair and nails; part of a B-complex vitamin regimen for metabolic health. | Choose for its direct, proven bioavailability and established role as the essential, active form of Vitamin B7 required for critical enzymatic functions. It is the universally recognized standard. |
Technical Documents
Available Documentation
COA
Safety Data Sheet (SDS)
Yes
Certificate of Analysis (COA)
Quality assurance documentation
Technical Data Sheet
Detailed technical specifications