Casein Phosphopeptide (CPP)
Nutritional Enhancers
691364-49-5
$20.84 ~ $31.26
Food
Free sample from 100g(NF)
One unit of:25kg/bag
One unit of:25kg/bag
25kg/bag
Product Info
What is Casein Phosphopeptide (CPP)?
Casein Phosphopeptide (CPP) is a bioactive peptide derived from milk casein primarily utilized in functional foods and dietary supplements to significantly enhance the absorption and bioavailability of essential minerals, especially calcium, iron, and zinc.
How is Casein Phosphopeptide (CPP) made?
| Step No. | Production Stage | Key Action | Control Point & Note |
|---|---|---|---|
| 1 | Raw Material Preparation | Disperse food-grade casein in purified water to form a uniform solution or slurry. | Control Point: Solution temperature (40-50°C) and pH (7.0-8.0) are critical to ensure complete dissolution and prepare the protein for the next step. |
| 2 | Enzymatic Hydrolysis | Add a specific protease enzyme (e.g., trypsin) to the casein solution under controlled conditions. | Control Point: This is the core step. Enzyme type/concentration, reaction time, temperature, and pH must be strictly controlled to achieve the desired degree of hydrolysis and maximize the yield of active CPPs. |
| 3 | Enzyme Inactivation | Rapidly heat the hydrolysate to a high temperature (e.g., 85-95°C) for a defined period. | Control Point: Precise time and temperature are vital to completely stop the enzymatic reaction, preventing over-hydrolysis which could destroy the target peptides. |
| 4 | Isoelectric Precipitation | Adjust the pH of the solution downwards (e.g., to pH 4.5) using a food-grade acid. | Control Point: The target pH causes unhydrolyzed casein and larger non-CPP peptides to precipitate out, while the smaller, soluble CPPs remain in the liquid phase. |
| 5 | Separation & Clarification | Remove the precipitated solids from the liquid using high-speed centrifugation or microfiltration. | Control Point: The goal is to obtain a clear supernatant, rich in CPP. The efficiency of the separation directly impacts the purity and final yield of the product. |
| 6 | Purification & Desalination | Process the clear supernatant through membrane filtration systems like ultrafiltration or nanofiltration. | Control Point: The membrane's molecular weight cut-off (MWCO) is key to selectively remove salts and free amino acids while retaining and concentrating the CPPs. |
| 7 | Decolorization & Deodorization | Treat the purified CPP solution with activated carbon, followed by filtration to remove the carbon. | Control Point: This optional step improves the final product's sensory attributes (color, taste, odor). Monitor carbon dosage and contact time to avoid removing target peptides. |
| 8 | Concentration | Evaporate excess water from the purified solution using a low-temperature vacuum evaporator. | Control Point: Increases the solid content to a level suitable for drying. Using low temperature and vacuum prevents heat damage and degradation of the heat-sensitive peptides. |
| 9 | Spray Drying | Atomize the concentrated CPP liquid into a chamber of hot air to instantly evaporate the remaining moisture. | Control Point: Inlet and outlet air temperatures are precisely controlled to produce a free-flowing powder with the correct moisture content and particle size, without denaturing the CPP. |
| 10 | Sieving & Packaging | Sieve the final powder for uniformity, conduct final quality control tests, and package into sealed, moisture-proof containers. | Control Point: Final QC tests confirm phosphorus content, peptide profile, and microbiological safety. Packaging is critical to ensure product stability and shelf-life. |
Technical Specifications
| CAS Number | 691364-49-5 |
| Solubility | Fully soluble in water across pH 4.5–7.0 |
| Storage Conditions | Store in cool dry place, <25 °C, sealed, protected from light |
| Shelf Life | 24 Months |
Applications & Usage
Common Applications:
Infant formula
dietary supplements
functional foods
beverages
dental-care products
nutraceuticals
Mechanism of action:
| Parameter | Casein Phosphopeptide (CPP) |
|---|---|
| Functional Category | Mineral Bioavailability Enhancer; Nutraceutical Agent |
| Key Ingredients | Bioactive phosphopeptides derived from enzymatic hydrolysis of casein, containing phosphoserine clusters (e.g., -Ser(P)-Ser(P)-Ser(P)-Glu-Glu-). |
| Mechanism of Action | Chelates divalent mineral cations (e.g., Ca2+, Fe2+, Zn2+) via phosphoserine residues to form soluble nano-complexes. This prevents the precipitation of minerals as insoluble salts (e.g., calcium phosphate) at the neutral pH of the small intestine, maintaining a high concentration of soluble minerals available for passive absorption across the intestinal epithelium. |
| Application Effect in Product | Increases the intestinal absorption and bioavailability of minerals in fortified foods (e.g., dairy, infant formula, beverages). Enhances the efficacy of mineral supplementation. Can improve dental health by stabilizing calcium and phosphate ions on the tooth surface, promoting remineralization. |
Comparison:
| Product Name | Category/Type | Key Features | Strengths (vs peers) | Weaknesses (vs peers) | Best Use Cases | Why Choose |
|---|---|---|---|---|---|---|
| Casein Phosphopeptide (CPP) | Milk-derived Bioactive Peptide | Forms soluble nano-complexes with minerals (Ca, Fe, Zn), preventing their precipitation in the intestine. | Enhances bioavailability of multiple minerals; unique anti-cavity properties by localizing calcium and phosphate on tooth enamel. | Derived from milk (potential allergen); less potent for specific minerals compared to dedicated chelates. | Calcium-fortified foods, bone health supplements, dental care products (toothpaste, mouthwash, chewing gum). | For broad mineral support in food systems and for dual-purpose dental health and remineralization applications. |
| Vitamin D3 (Cholecalciferol) | Fat-soluble Vitamin | Acts as a hormone to upregulate intestinal transporters for calcium and phosphate absorption. | Essential nutrient with systemic effects on bone and immune health; highly effective and well-researched for calcium regulation. | Primarily targets calcium and phosphate; ineffective if dietary mineral intake is insufficient; potential for toxicity at high doses. | General supplements for bone health, fortification of dairy and cereals, correcting vitamin D deficiency. | To address the hormonal regulation side of calcium absorption, especially when deficiency is a concern. |
| Prebiotics (FOS/Inulin) | Soluble Dietary Fiber | Fermented by gut bacteria into SCFAs, which lower colonic pH and increase mineral solubility and absorption. | Provides dual benefits of improved gut health and mineral absorption; plant-based and non-allergenic. | Indirect mechanism; can cause GI distress (gas, bloating) in some individuals; efficacy depends on individual gut microbiome. | Fiber-enriched functional foods, synbiotic supplements (with probiotics), products targeting overall digestive wellness. | For a holistic approach that improves gut health while also providing a modest boost in mineral absorption. |
| Amino Acid Chelates | Mineral Chelate | Binds a mineral ion to one or more amino acids, allowing it to be absorbed through amino acid pathways. | Extremely high bioavailability; stable and non-reactive in the gut, leading to fewer GI side effects (e.g., constipation). | Higher cost per unit of mineral; can be bulkier, requiring larger pills for the same elemental dose. | High-potency mineral supplements, formulations for individuals with malabsorption issues or sensitive stomachs. | For maximum, targeted absorption of a specific mineral with minimal gastrointestinal discomfort. |
| Lactoferrin | Milk-derived Glycoprotein | Binds iron with very high affinity, protecting it through the digestive tract and facilitating its absorption. | Highly specific and effective for iron absorption; also possesses valuable antimicrobial and immunomodulatory properties. | Primarily focused on iron; less impact on other minerals like calcium; higher cost and potential milk allergen. | Infant formula, supplements for iron-deficiency anemia, products targeting both immune support and iron status. | When the primary goal is to increase iron bioavailability, especially when added immune benefits are also desired. |
Technical Documents
Available Documentation
COA, spec sheet, regulatory & allergen docs
Safety Data Sheet (SDS)
SDS available upon request
Certificate of Analysis (COA)
Quality assurance documentation
Technical Data Sheet
Detailed technical specifications