L-Hydroxyproline
Amino Acids
Nutritional Enhancers
51-35-4
C₅H₉NO₃
$26.68 ~ $40.02
Food
Free sample from 100g(NF)
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One unit of:Unknown
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Product Info
What is L-Hydroxyproline?
L-Hydroxyproline is a non-essential amino acid, formed primarily by the modification of proline, that is a fundamental constituent of collagen and is often used in nutritional supplements for connective tissue support.
How is L-Hydroxyproline made?
| Step No. | Production Stage | Key Action | Control Point & Note |
|---|---|---|---|
| 1 | Raw Material Preparation | Sourcing and preparing high-quality, collagen-rich raw material (e.g., food-grade gelatin, animal hides). | Control Point: Source and purity of the collagen. Note: Material must be verified to be free from contaminants and meet food or pharmaceutical standards. |
| 2 | Acid Hydrolysis | Breaking down the collagen protein into its constituent amino acids using a strong acid (e.g., hydrochloric acid) under heat. | Control Point: Acid concentration, temperature (e.g., 110-120°C), and reaction time. Note: These parameters are critical for achieving complete hydrolysis while minimizing amino acid degradation. |
| 3 | Neutralization & Filtration | Adjusting the pH of the acidic hydrolysate to a neutral range (pH 6.5-7.0) using an alkaline solution, causing salts to precipitate. | Control Point: Final pH level and temperature. Note: The solution is then filtered to remove the precipitated salts and other insoluble matter. |
| 4 | Decolorization | Treating the amino acid solution with activated carbon to adsorb colored impurities and other organic residues. | Control Point: Quantity of activated carbon and contact time. Note: This step significantly improves the clarity and color of the final product. |
| 5 | Ion Exchange Chromatography | Passing the solution through a column packed with ion-exchange resin to separate L-Hydroxyproline from other amino acids. | Control Point: Flow rate, pH, and column temperature. Note: This is the most crucial purification step that determines the final product's purity. Different amino acids are eluted at different times. |
| 6 | Concentration & Crystallization | Concentrating the purified L-Hydroxyproline fraction under vacuum to increase saturation, followed by controlled cooling to induce crystallization. | Control Point: Vacuum level and cooling rate. Note: Proper control ensures high yield and uniform crystal size. |
| 7 | Centrifugation & Washing | Separating the solid crystals from the mother liquor using a centrifuge. The collected crystals are then washed with purified water. | Control Point: Centrifuge speed and purity of the wash water. Note: Washing removes residual impurities adhering to the crystal surface. |
| 8 | Drying | Drying the washed crystals in a vacuum dryer at a controlled temperature to remove residual moisture. | Control Point: Drying temperature and time. Note: Must achieve a specific moisture content (e.g., <0.5%) without causing thermal degradation of the product. |
| 9 | Sieving & Blending | Milling and sieving the dried product to achieve a consistent and specified particle size distribution. | Control Point: Sieve mesh size. Note: Ensures product homogeneity and meets customer specifications for powder fineness. |
| 10 | Quality Control & Packaging | Performing final quality analysis (purity, heavy metals, microbial content) and packaging the product into sealed, labeled containers in a cleanroom. | Control Point: Final product assay and packaging integrity. Note: All specifications must be met before release. Proper packaging prevents contamination and moisture uptake. |
Technical Specifications
| CAS Number | 51-35-4 |
| Chemical Formula | C₅H₉NO₃ |
| Solubility | Freely soluble in water |
| Storage Conditions | Keep tightly closed in a dry, cool, and ventilated place |
| Shelf Life | 24 Months |
Applications & Usage
Common Applications:
Collagen synthesis support
pharmaceutical and cosmetic formulations
nutritional supplements
Mechanism of action:
| Parameter | L-Hydroxyproline |
|---|---|
| Functional Category | Nutritional Supplement; Flavor Enhancer; Texture Stabilizer |
| Key Ingredients | L-4-Hydroxyproline (an amino acid) |
| Mechanism of Action | As a primary constituent of collagen, it stabilizes protein structures via hydrogen bonding within the polypeptide triple helix. When added exogenously, it participates in Maillard reactions with reducing sugars to generate savory and meat-like flavor notes. It also serves as a precursor for endogenous collagen synthesis, supporting connective tissue integrity. |
| Application Effect in Product | Enhanced savory, roasted, or brothy flavor profiles in soups, sauces, and seasonings. Improved textural stability and firmness in gelatin-based desserts and processed meat products. Provides nutritional value as a key component for collagen and connective tissue synthesis. |
Comparison:
| Product Name | Category/Type | Key Features | Strengths (vs peers) | Weaknesses (vs peers) | Best Use Cases | Why Choose |
|---|---|---|---|---|---|---|
| L-Hydroxyproline | Non-proteinogenic amino acid | A major component of collagen; provides structural stability to the collagen triple helix. | Provides the final, hydroxylated form directly, bypassing the enzymatic conversion step from proline. | Less common as a standalone supplement; the body can synthesize it from L-proline with sufficient Vitamin C. | Targeted supplementation for connective tissue; scientific research on collagen synthesis. | To directly supply a key finished component of collagen, potentially useful if proline conversion is inefficient. |
| L-Proline | Proteinogenic amino acid | Direct precursor to L-Hydroxyproline; a fundamental building block for all proteins. | More versatile than hydroxyproline as it's used in many biological processes; widely available. | Requires conversion to hydroxyproline, a step dependent on cofactors like Vitamin C, iron, and oxygen. | General support for collagen production for skin, joints, and tendons, especially when paired with Vitamin C. | To provide the body with the primary raw material to regulate its own hydroxyproline production. |
| Glycine | Proteinogenic amino acid | The most abundant amino acid in collagen, making up roughly one-third of its structure. | Addresses the largest quantitative amino acid need for collagen synthesis; has additional benefits for sleep and neurotransmission. | Is only one of several key amino acids needed; does not provide proline or hydroxyproline. | Supporting overall collagen matrix volume; improving sleep quality; general amino acid supplementation. | To ensure an ample supply of the most critical amino acid by volume, which can be a rate-limiting factor. |
| Collagen Peptides | Protein Derivative / Peptide Mix | Pre-digested, highly bioavailable peptides containing the full spectrum of collagen amino acids, including hydroxyproline. | Provides all necessary building blocks (glycine, proline, hydroxyproline) in their natural ratios. Very easy to absorb. | More expensive than individual amino acids; typically derived from animal sources (bovine, marine, etc.). | Comprehensive support for skin, hair, nails, and joints; post-workout recovery for connective tissues. | For an all-in-one, complete solution that delivers every required collagen building block in a highly absorbable form. |
| Vitamin C (Ascorbic Acid) | Essential Vitamin / Cofactor | An essential cofactor for the enzymes that convert proline to hydroxyproline and lysine to hydroxylysine. | Absolutely critical for collagen synthesis; without it, the process halts. Provides broad antioxidant benefits. | It is not a structural building block; ineffective for collagen synthesis without adequate amino acid intake. | Paired with any collagen-building amino acid or peptide; addressing dietary insufficiency; general immune support. | To enable the crucial enzymatic reaction that creates stable collagen. It makes the amino acid building blocks functional. |
Technical Documents
Available Documentation
COA, TDS available
Safety Data Sheet (SDS)
MSDS available
Certificate of Analysis (COA)
Quality assurance documentation
Technical Data Sheet
Detailed technical specifications