Phytase
Enzyme Preparations
Nutritional Enhancers
3.1.3.8
$5.08 ~ $7.62
Food
Free sample from 100g(NF)
One unit of:25kg/barrel
One unit of:25kg/barrel
25kg/barrel
Product Info
What is Phytase?
Phytase is an enzyme that hydrolyzes indigestible phytic acid (phytate), primarily used in animal feed to increase the bioavailability of phosphorus and reduce environmental pollution.
How is Phytase made?
| Step No. | Production Stage | Key Action | Control Point & Note |
|---|---|---|---|
| 1 | Inoculum Preparation | Cultivate a high-yield microbial strain (e.g., Aspergillus niger or Pichia pastoris) in a seed fermenter. | Strain Purity & Viability: Aseptic techniques are essential to prevent contamination. The inoculum must be healthy and active to ensure a successful main fermentation. |
| 2 | Medium Formulation | Prepare and sterilize the nutrient-rich liquid medium for the main fermenter. | Nutrient Composition: Control levels of carbon, nitrogen, and minerals. Low phosphate concentration is a critical parameter used to induce phytase gene expression. Sterilization must be complete. |
| 3 | Submerged Fermentation | Inoculate the sterile medium with the seed culture and run the fermentation process in a large-scale bioreactor. | Process Parameters: Tightly control pH, Temperature, Dissolved Oxygen (DO), and Agitation Speed for optimal enzyme secretion. Fermentation time is determined by monitoring enzyme activity. |
| 4 | Harvest & Cell Separation | Separate the microbial biomass (cells) from the liquid culture broth containing the secreted phytase enzyme. | Separation Efficiency: Use centrifugation or microfiltration to obtain a clear, cell-free supernatant. The goal is to maximize the recovery of the extracellular enzyme. |
| 5 | Concentration | Concentrate the enzyme-rich supernatant to reduce volume and increase enzyme concentration. | Ultrafiltration: Use a membrane with an appropriate Molecular Weight Cut-Off (MWCO) to retain the phytase protein while allowing water and smaller solutes to pass through. |
| 6 | Formulation & Stabilization | Mix the concentrated enzyme liquid with stabilizing agents and carriers (e.g., starch, maltodextrin). | Stabilizer Choice & Dosage: The formulation is critical for maintaining enzyme stability during drying and subsequent storage. It protects the enzyme from heat and moisture. |
| 7 | Drying & Granulation | Convert the liquid formulation into a solid, free-flowing, and stable granular product. | Drying Temperature: Use low-temperature methods like fluid-bed granulation or spray drying to prevent thermal denaturation of the enzyme. Final moisture content is a key control point. |
| 8 | Final Quality Control & Packaging | Test the final granules for activity, moisture, and purity, then package into sealed containers. | Enzyme Activity Assay (FTU/g): This is the most critical quality specification. Also check for particle size and microbial contamination. Package in moisture-proof containers to ensure shelf life. |
Technical Specifications
| CAS Number | 3.1.3.8 |
| Solubility | Soluble in water or buffer |
| Storage Conditions | Store cool, dry; 2–8 °C |
| Shelf Life | 24 Months |
Applications & Usage
Common Applications:
Degrades phytate to improve phosphorus/mineral bioavailability in food & feed
used in baking
feed
food processing
Mechanism of action:
| Parameter | Phytase |
|---|---|
| Functional Category | Enzyme; Processing Aid; Mineral Bioavailability Enhancer |
| Key Ingredients | Phytase (myo-inositol hexakisphosphate phosphohydrolase), typically derived from microbial sources like Aspergillus niger or Trichoderma reesei. |
| Mechanism of Action | Catalyzes the stepwise hydrolysis of phytic acid (myo-inositol hexakisphosphate), an anti-nutritional factor found in plant-based ingredients like grains and legumes. This enzymatic action releases inositol and inorganic phosphate, thereby breaking the chelate complexes that phytic acid forms with essential dietary minerals (e.g., Ca²⁺, Zn²⁺, Fe²⁺, Mg²⁺) and proteins. |
| Application Effect in Product | Increases the bioavailability of essential minerals in plant-based foods and animal feed, improving nutritional value. Reduces the need for mineral supplementation. In baking, can improve dough machinability, loaf volume, and crumb structure by reducing the inhibitory effects of phytate on native amylases and proteases. |
Comparison:
| Product Name | Category/Type | Key Features | Strengths (vs peers) | Weaknesses (vs peers) | Best Use Cases | Why Choose |
|---|---|---|---|---|---|---|
| Phytase | Feed Enzyme | Hydrolyzes phytic acid in plant ingredients to release digestible phosphorus (P) and other minerals. | Reduces need for inorganic P supplements, lowers feed cost, decreases environmental P pollution, mitigates anti-nutritional effects of phytate. | Efficacy can be reduced by high heat during feed processing; requires precise formulation to be effective. | Diets for monogastric animals (poultry, swine) high in plant-based ingredients like corn, soy, and wheat. | To improve nutrient availability from existing feedstuffs, reduce costs, and minimize environmental impact. |
| Monocalcium Phosphate (MCP) | Inorganic Feed Phosphate | A mined and processed mineral providing a direct source of highly bioavailable phosphorus and calcium. | Highly reliable and predictable source of P and Ca; not sensitive to feed processing conditions; simple to formulate with. | Non-renewable resource; typically higher cost inclusion than using phytase; does not address other anti-nutritional effects of phytate. | Any animal diet needing supplemental P and Ca, especially when formulation simplicity and guaranteed delivery are prioritized. | For a direct, guaranteed supply of phosphorus and calcium, bypassing the complexities of enzyme activity. |
| Dicalcium Phosphate (DCP) | Inorganic Feed Phosphate | A mineral providing both phosphorus and calcium to animal diets. | Provides both P and Ca reliably; stable during feed processing. | Phosphorus has lower bioavailability than in MCP; non-renewable resource; adds to feed cost without addressing phytate issues. | General use in livestock feeds, though often replaced by MCP in high-performance monogastric diets due to lower P digestibility. | When a basic, reliable inorganic source of both P and Ca is needed, and maximum P bioavailability is not the primary driver. |
| Xylanase | Feed Enzyme (NSP Enzyme) | Breaks down non-starch polysaccharides (NSPs) like arabinoxylans in cereal grains. | Improves energy utilization and overall nutrient digestibility by reducing gut viscosity; works synergistically with phytase. | Does not release phosphorus from phytate; its benefit is dependent on the specific grains used in the diet (e.g., wheat, rye). | Poultry and swine diets containing high levels of viscous grains like wheat, barley, or rye. | To increase the energy value of the feed and improve gut health. It is a complementary enzyme, not a substitute for phytase. |
Technical Documents
Available Documentation
COA, SDS available
Safety Data Sheet (SDS)
Available
Certificate of Analysis (COA)
Quality assurance documentation
Technical Data Sheet
Detailed technical specifications