Nattokinase (Subtilisin NAT)
Enzyme Preparations
Nutritional Enhancers
$13.82 ~ $20.73
Food
Free sample from 100g(NF)
One unit of:17kg/barrel
One unit of:17kg/barrel
17kg/barrel
Product Info
What is Nattokinase (Subtilisin NAT)?
Nattokinase (Subtilisin NAT) is a potent, fibrin-dissolving enzyme isolated from the traditional Japanese food natto (fermented soybeans) and is widely utilized in dietary supplements to support cardiovascular health and healthy circulation.
How is Nattokinase (Subtilisin NAT) made?
| Step No. | Production Stage | Key Action | Control Point & Note |
|---|---|---|---|
| 1 | Raw Material Preparation | Select, soak, and steam high-quality, non-GMO soybeans. | Control Point: Sterility of soybeans and equipment; cooking time and temperature. Note: This step eliminates competing microorganisms and makes soybean nutrients accessible for bacterial growth. |
| 2 | Inoculation | Introduce a pure, high-activity strain of Bacillus subtilis var. natto to the cooled, steamed soybeans. | Control Point: Purity and viability of the bacterial culture; inoculation ratio and temperature. Note: Using a proprietary, high-yield strain is critical for efficient enzyme production. |
| 3 | Fermentation | Incubate the inoculated soybeans under precisely controlled conditions. | Control Point: Temperature (38-42°C), humidity, aeration, and fermentation time (24-48 hours). Note: This is the core bioprocess where the bacteria produce and secrete the nattokinase enzyme. |
| 4 | Extraction | Harvest the fermented mass (natto) and use a buffer solution to extract the crude enzyme. | Control Point: Buffer pH, temperature, and extraction duration. Note: The goal is to solubilize the maximum amount of active nattokinase from the solid fermented material. |
| 5 | Purification & Clarification | Separate the liquid extract from solid residues using centrifugation and microfiltration. | Control Point: Centrifugal force (G-force); filter pore size. Note: This step removes insoluble soybean parts and bacterial cells, resulting in a clarified crude enzyme solution. |
| 6 | Concentration & Isolation | Process the clarified solution through ultrafiltration and chromatography. | Control Point: Membrane molecular weight cutoff (MWCO); chromatography column type and elution conditions. Note: This isolates the nattokinase protein from other smaller molecules and impurities, significantly increasing its purity and concentration. |
| 7 | Standardization | Assay the enzyme's activity in Fibrinolytic Units (FU) and blend with an excipient (e.g., maltodextrin). | Control Point: Accuracy of the FU/g assay; precise blending ratios. Note: This crucial step ensures that the final product has a consistent and guaranteed potency per gram. |
| 8 | Drying | Convert the liquid concentrate into a stable powder using freeze-drying (lyophilization). | Control Point: Freezing temperature, vacuum pressure, and drying cycle time. Note: Freeze-drying is the preferred method as it preserves the delicate three-dimensional structure and biological activity of the enzyme. |
| 9 | Final QC & Packaging | Mill, sieve, and conduct final quality control tests on the finished powder before packaging. | Control Point: Final activity (FU/g), purity, microbial limits, heavy metals, and moisture content. Note: Rigorous final testing confirms the product meets all food-grade or pharmaceutical-grade specifications before being sealed in moisture-proof packaging. |
Technical Specifications
| Solubility | Soluble in water or gastric fluid |
| Storage Conditions | Store cool & dry (2–25 °C) |
| Shelf Life | 24 Months |
Applications & Usage
Common Applications:
Dietary supplement: fibrinolytic
support cardiovascular health
potential Alzheimer’s adjunct
Mechanism of action:
| Parameter | Nattokinase (Subtilisin NAT) |
|---|---|
| Functional Category | Enzymatic Processing Aid; Fibrinolytic Agent; Bioactive Compound |
| Key Ingredients | Nattokinase (a serine protease enzyme, EC 3.4.21.62) derived from fermentation of soybeans with Bacillus subtilis var. natto. |
| Mechanism of Action | Exhibits potent fibrinolytic (anti-clotting) activity by directly hydrolyzing fibrin, a key protein in blood clot formation. It also activates pro-urokinase and tissue plasminogen activator (t-PA), increasing the body's natural production of plasmin, which further degrades fibrin. As a broad-spectrum protease, it can also hydrolyze various food proteins by cleaving peptide bonds. |
| Application Effect in Product | Primarily used in dietary supplements to support cardiovascular health by promoting healthy blood circulation and pressure. Can be used experimentally in food systems to tenderize protein-rich products or modify dough rheology by controlled proteolysis of gluten or other structural proteins. |
Comparison:
| Product Name | Category/Type | Key Features | Strengths (vs peers) | Weaknesses (vs peers) | Best Use Cases | Why Choose |
|---|---|---|---|---|---|---|
| Nattokinase (Subtilisin NAT) | Fibrinolytic Enzyme | Derived from fermented soybeans (natto); directly dissolves fibrin; measured in fibrinolytic units (FU). | Strong, direct fibrinolytic action; good oral bioavailability; extensive research for a supplement. | Contains Vitamin K2, which can interact with warfarin; potential soy allergen. | Supporting healthy circulation, managing blood viscosity, and addressing abnormal fibrin buildup. | For a potent, well-researched natural enzyme with direct clot-dissolving properties. |
| Lumbrokinase | Fibrinolytic Enzyme Complex | Derived from earthworms; a complex of multiple fibrin-dissolving enzymes. | Very high potency, often considered stronger than nattokinase; free of Vitamin K2. | Significantly more expensive; less widely available and researched than nattokinase. | Aggressive fibrinolytic support, especially when avoiding Vitamin K2 is critical. | For maximum enzyme potency without interference with certain anticoagulant drugs. |
| Serrapeptase | Proteolytic Enzyme | Derived from silkworms; breaks down non-living tissue, inflammation byproducts, and mucus. | Excellent anti-inflammatory properties; effective for pain, swelling, and sinus issues. | Weaker direct fibrinolytic activity compared to nattokinase or lumbrokinase. | Conditions involving chronic inflammation, pain, scar tissue, or excess mucus. | When the primary goal is reducing inflammation and pain, with a secondary circulatory benefit. |
| Aspirin (low-dose) | Antiplatelet Drug (NSAID) | Synthetic medication that inhibits platelet aggregation (clumping). | Very inexpensive; vast body of clinical evidence for cardiovascular event prevention. | Does not dissolve existing clots (fibrin); risk of gastrointestinal bleeding; different mechanism of action. | Medically supervised prevention of heart attack and stroke in at-risk populations. | To follow a standard, evidence-based medical protocol for preventing platelet-driven clots. |
| Bromelain | Proteolytic Enzyme | Derived from pineapple; primarily known for anti-inflammatory and digestive effects. | Supports digestion; effective for reducing swelling from injuries; widely available. | Very mild fibrinolytic effect; primarily an anti-inflammatory and digestive aid. | Post-injury swelling and bruising, digestive support, general inflammation. | For a gentle, multi-purpose enzyme focused on digestion and minor inflammation, not circulation. |
Technical Documents
Available Documentation
COA, SDS available
Safety Data Sheet (SDS)
Available
Certificate of Analysis (COA)
Quality assurance documentation
Technical Data Sheet
Detailed technical specifications